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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which leads to chromosome instability, and thus could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two added splice variants had been claimed but in depth nucleotide sequence info will not be but out there.
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Blocking peptides are peptides that bind specially for the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This manual outlines further doable optimization of cellular and immunoassay parameters to make certain the best possible effects are...
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This guidebook outlines more possible optimization of mobile and immunoassay parameters to ensure the best possible final results are...
Antibodies bound to the blocking peptide no longer bind to the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Target Facts Cyclin E belongs into the extremely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates with the G1-S period boundary and is also degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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